axio vision software (version 4.8) Search Results


microscope  (Carl Zeiss)
99
Carl Zeiss microscope
Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microscope/product/Carl Zeiss
Average 99 stars, based on 1 article reviews
microscope - by Bioz Stars, 2026-04
99/100 stars
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48-well  (Axion BioSystems)
90
Axion BioSystems 48-well
48 Well, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/48-well/product/Axion BioSystems
Average 90 stars, based on 1 article reviews
48-well - by Bioz Stars, 2026-04
90/100 stars
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axio observer d1 inverted microscope  (Carl Zeiss)
96
Carl Zeiss axio observer d1 inverted microscope
Axio Observer D1 Inverted Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axio observer d1 inverted microscope/product/Carl Zeiss
Average 96 stars, based on 1 article reviews
axio observer d1 inverted microscope - by Bioz Stars, 2026-04
96/100 stars
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lumos optical stimulator  (Axion BioSystems)
90
Axion BioSystems lumos optical stimulator
Lumos Optical Stimulator, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lumos optical stimulator/product/Axion BioSystems
Average 90 stars, based on 1 article reviews
lumos optical stimulator - by Bioz Stars, 2026-04
90/100 stars
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48-well mea plates  (Axion BioSystems)
90
Axion BioSystems 48-well mea plates
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
48 Well Mea Plates, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/48-well mea plates/product/Axion BioSystems
Average 90 stars, based on 1 article reviews
48-well mea plates - by Bioz Stars, 2026-04
90/100 stars
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mea plate lumos 48  (Axion BioSystems)
90
Axion BioSystems mea plate lumos 48
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Mea Plate Lumos 48, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mea plate lumos 48/product/Axion BioSystems
Average 90 stars, based on 1 article reviews
mea plate lumos 48 - by Bioz Stars, 2026-04
90/100 stars
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axio imager m1 microscope  (Carl Zeiss)
90
Carl Zeiss axio imager m1 microscope
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Axio Imager M1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axio imager m1 microscope/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
axio imager m1 microscope - by Bioz Stars, 2026-04
90/100 stars
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inverted phase contrast photomicroscope  (Carl Zeiss)
96
Carl Zeiss inverted phase contrast photomicroscope
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Inverted Phase Contrast Photomicroscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverted phase contrast photomicroscope/product/Carl Zeiss
Average 96 stars, based on 1 article reviews
inverted phase contrast photomicroscope - by Bioz Stars, 2026-04
96/100 stars
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axio observer  (Carl Zeiss)
90
Carl Zeiss axio observer
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Axio Observer, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axio observer/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
axio observer - by Bioz Stars, 2026-04
90/100 stars
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inverted widefield fluorescence microscope zeiss axio observer  (Carl Zeiss)
90
Carl Zeiss inverted widefield fluorescence microscope zeiss axio observer
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Inverted Widefield Fluorescence Microscope Zeiss Axio Observer, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverted widefield fluorescence microscope zeiss axio observer/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
inverted widefield fluorescence microscope zeiss axio observer - by Bioz Stars, 2026-04
90/100 stars
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axio vert.a1 microscope  (Carl Zeiss)
90
Carl Zeiss axio vert.a1 microscope
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Axio Vert.A1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axio vert.a1 microscope/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
axio vert.a1 microscope - by Bioz Stars, 2026-04
90/100 stars
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48-well mea plates axion m768-kap-48  (Axion BioSystems)
90
Axion BioSystems 48-well mea plates axion m768-kap-48
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
48 Well Mea Plates Axion M768 Kap 48, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/48-well mea plates axion m768-kap-48/product/Axion BioSystems
Average 90 stars, based on 1 article reviews
48-well mea plates axion m768-kap-48 - by Bioz Stars, 2026-04
90/100 stars
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( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) MEA experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 MEA plates for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.

Journal: eLife

Article Title: Alzheimer’s disease risk gene BIN1 induces Tau-dependent network hyperexcitability

doi: 10.7554/eLife.57354

Figure Lengend Snippet: ( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) MEA experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 MEA plates for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.

Article Snippet: For 48-well plate multielectrode array recordings, neurons were plated at 30,000 per well in 48-well MEA plates (Axion Biosystems, M768-tMEA-48B-5).

Techniques: In Vitro, Immunostaining, Fluorescence, Activity Assay, MANN-WHITNEY, Expressing